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分析

by Le Thi Anh Hong, Joachim Clemens 和 Nguyen Thai Hoa.


一个水质样本中监测出了雌性激素并通过了是非检测(Routledge and Sumpter, 1996),该检测利用了吸收微孔板光度计(利用Elisa读数法读取540nm处和630nm处的吸光度)。图1显示了内分泌干扰因子对酵母细胞的反应,图2是17β-雌二醇(17β- estradiol)在0.1μg/L至3.5 μg/L 浓度范围内的的标准曲线图。


 



图1:酵母细胞对内分泌干扰物的反应


 



图2:17β-雌二醇标准曲线图


 


与农药残量监测工作组的紧密合作,我们分析了一组农药的动情性(丙环唑(Propiconazole)、α-磺胺类药剂(alpha endosulphan)、β-磺胺类药剂(beta endosulphan)、甲胺磷(methamidophos)、乐果(dimethoate)、二嗪农(diazinon)、α-氯氰菊酯(alpha cypermethrin)、锐劲特(Fipronil)和吡虫啉(Imidacloprid))。其中,丙环唑(Propiconazole)和锐劲特(Fipronil)呈阳性反应。


 


 


文献


 


Routledge EJ, Sumpter JP (1996): Estrogenic activity of surfactants and some of their degradation products assessed using a recombinant yeast screen; environmental Toxicology and Chemistry, Vol. 15 ,No. 3, 241-248.


 


Sonoko Yamaguchi, Aki Ito, Toshitsugu Higashino, Chiemi Miura, Tetsuro Agusa, Reiji Kubota, Hisato Iwata, Shinsuke Tanabe, and Takeshi Miura (2004). Influence of endocrine disruptors on reproductive of aquatic animals in Indochina.


 


Proceedings of the International Symposium on the Development of Water Resource Management System in Mekong Watershed, December 3rd-4th, 2004, Hanoi, Vietnam, pp 32-40.


 

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